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[advancedbiomatrix] Type I Collagen Solution, 6 mg/ml (Bovine)

작성자 관리자 작성일 2021-10-29
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  카탈로그 NO :#5010
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  원산지 :미국
  생산기업 :advancedbiomatrix
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Nutragen®

Type I Collagen Solution, 6 mg/ml (Bovine)

Catalog #5010

Nutragen® is a 6 mg/ml, type I bovine atelocollagen solution for 2D and 3D cell culture. Due to having a higher concentration, Nutragen® hydrogels will be stronger than other lower concentration collagen products.

Product Description

 

Nutragen® collagen is known as the standard of all collagens for purity (>99% collagen content), functionality, and the most native-like collagen available.  Nutragen®  is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

Nutragen® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. Nutragen® collagen is supplied at approximately a 6 mg/mL concentration to provide an extremely firm gel.  The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product.  Nutragen® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

Nutragen® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.  Nutragen®  collagen is provided in a 50 mL volume and is contained in user-friendly packaging for use and storage.  Nutragen®  is sterile filtered and is supplied as a ready to use solution.

Parameter, Testing, and MethodNutragen® Type I Collagen #5010
Sterilization MethodFiltration
Extraction MethodEnzyme - atelocollagen
FormSolution
Package Size50 mL, 1000 mL
Storage Temperature2-10°C
Shelf LifeMinimum of 6 months from date of receipt

Collagen Concentration - Biuret

5.8-6.8 mg/mL

Collagen Purity - Silver Staining

>99%
pH1.9-2.1
Kinetic Gel Test (Minutes)<40
Gel Formation Tube Test (Minutes)<40

Fibrillogenesis (Absorbance Units)

>0.5

Electrophoretic Pattern - Coomassie Blue

Characteristic
Sterility - USP modifiedNo growth
Endotoxin - LAL<1.0 EU/mL
Osmolality (mOsmo H2O/kg)<35
Cell Attachment AssayPass
SourceBovine Hide
Hydrogel Young's Modulus E (Pa)Characteristic

 

Product References

 

References for Nutragen®:

Tanic, J., et al. "Adseverin modulates morphology and invasive function of MCF7 cells." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2019).

Rosell-García, Tamara, et al. "Differential cleavage of lysyl oxidase by the metalloproteinases BMP1 and ADAMTS2/14 regulates collagen binding through a tyrosine sulfate domain." Journal of Biological Chemistry (2019): jbc-RA119.

Berger, Anthony J. Decoupling the Properties of the Physical Microenvironment: The Design and Application of a Gelatin-Methacrylate/Collagen Composite Hydrogel. Diss. The University of Wisconsin-Madison, 2018.

Berger, Anthony J. Decoupling the Properties of the Physical Microenvironment: The Design and Application of a Gelatin-Methacrylate/Collagen Composite Hydrogel. Diss. The University of Wisconsin-Madison, 2018.

Renkawitz, Jörg, et al. "Nuclear positioning facilitates amoeboid migration along the path of least resistance." Nature568.7753 (2019): 546.

Szulc, Daniel Andrzej, and Hai‐Ling Margaret Cheng. "One‐Step Labeling of Collagen Hydrogels with Polydopamine and Manganese Porphyrin for Non‐Invasive Scaffold Tracking on Magnetic Resonance Imaging." Macromolecular bioscience(2019): 1800330.

Formica, Florian A., Goncalo Barreto, and Marcy Zenobi-Wong. "Cartilage-targeting dexamethasone prodrugs increase the efficacy of dexamethasone." Journal of Controlled Release295 (2019): 118-129.

Dubois, Fatéméh, et al. "A role for RASSF1A in tunneling nanotube formation between cells through GEFH1/Rab11 pathway control." Cell Communication and Signaling 16.1 (2018): 66.

Čermák, Vladimír, et al. "RNA-seq of macrophages of amoeboid or mesenchymal migratory phenotype due to specific structure of environment." Scientific data 5 (2018): 180198.

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